Rat IP-10/CXCL10 (Interferon Gamma Induced Protein 10kDa) ELISA Kit
The Rat IP-10/CXCL10 (Interferon Gamma Induced Protein 10kDa) ELISA Kit is a specialized assay designed for the accurate quantitative measurement of Interferon Gamma Induced Protein 10kDa levels in a variety of biological samples.
IP-10, also known as CXCL10, is a key chemokine involved in immune responses, particularly regulated by interferon gamma in inflammatory processes. This cytokine plays a crucial role in recruiting immune cells to sites of infection and inflammation and is significant in various immune-related disorders and infections. Accurate measurement of IP-10/CXCL10 levels is vital for elucidating its role in immune responses, autoimmune diseases, and infectious conditions. The Rat IP-10/CXCL10 ELISA Kit from Assay Genie provides exceptional sensitivity and specificity, ensuring reliable and reproducible results. With stringent quality control standards, this kit offers robust performance and user-friendly procedures, making it an excellent choice for cutting-edge research in immunology and inflammatory diseases.
Product Name:
Rat IP-10/CXCL10 (Interferon Gamma Induced Protein 10kDa) ELISA Kit
SKU:
AEES00449
Target:
Rat IP-10/CXCL10 (Interferon Gamma Induced Protein 10kDa)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IP-10/CXCL10. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IP-10/CXCL10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IP-10/CXCL10, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat IP-10/CXCL10. You can calculate the concentration of Rat IP-10/CXCL10 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-102
87-99
91-103
Average (%)
93
93
96
1:4
Range (%)
94-109
89-102
93-107
Average (%)
100
94
99
1:8
Range (%)
100-114
83-95
94-108
Average (%)
107
89
99
1:16
Range (%)
99-112
88-100
97-111
Average (%)
105
94
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-105
98
EDTA plasma (n=5)
87-98
92
Cell culture media (n=5)
91-103
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
93.9
203.3
880.9
93.0
209.3
921.5
Standard deviation
5.2
11.8
36.1
5.4
11.1
38.7
C V (%)
5.54
5.8
4.1
5.81
5.3
4.2
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat IP-10/CXCL10 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat IP-10/CXCL10 in samples. No significant cross-reactivity or interference between Rat IP-10/CXCL10 and analogues was observed.
