The Rat Kidney Injury Molecule 1 (KIM-1) ELISA Kit is a specialized assay designed for the quantitative measurement of KIM-1 levels in various biological samples obtained from rats. KIM-1, also known as T cell immunoglobulin mucin-1 (TIM-1), is a well-known biomarker for kidney injury and renal damage, playing a crucial role in the early detection of kidney injury and monitoring kidney function. This ELISA kit enables researchers to accurately measure and quantify KIM-1 concentrations, providing valuable insights into kidney injury, renal function, and nephrotoxicity. By offering exceptional sensitivity and specificity, this kit allows for precise and reliable assessment of KIM-1 levels, which is essential for understanding renal pathology, evaluating kidney health, and developing targeted therapeutic strategies for kidney-related disorders.
Manufactured under stringent quality control standards, this ELISA kit by Assay Genie delivers robust performance and reproducible results, ensuring accurate and consistent KIM-1 level measurements. With its user-friendly protocols and high-performance characteristics, this kit is an ideal choice for research studies investigating kidney injury, renal biomarkers, and the impact of nephrotoxicity in rat models.
Product Name:
Rat KIM-1 (Kidney Injury Molecule 1) ELISA Kit
SKU:
AEES00533
Target:
Rat KIM-1 (Kidney Injury Molecule 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat KIM-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat KIM-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat KIM-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat KIM-1. You can calculate the concentration of Rat KIM-1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-102
98-107
91-101
Average (%)
96
103
94
1:4
Range (%)
90-98
87-97
92-100
Average (%)
95
90
96
1:8
Range (%)
98-106
89-101
85-98
Average (%)
103
93
91
1:16
Range (%)
94-108
95-108
90-101
Average (%)
101
103
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-99
92
EDTA plasma (n=5)
87-101
95
Cell culture media (n=5)
90-101
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.92
2.3
8.98
0.97
2.38
9.6
Standard deviation
0.05
0.11
0.49
0.05
0.11
0.45
C V (%)
5.43
4.78
5.46
5.15
4.62
4.69
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat KIM-1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat KIM-1 in samples. No significant cross-reactivity or interference between Rat KIM-1 and analogues was observed.
