The Rat LEP (Leptin) CLIA Kit is specially designed for the quantitative detection of leptin levels in rat serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research applications.Leptin is a key hormone that plays a vital role in regulating energy balance and body weight. It is involved in controlling appetite, metabolism, and fat storage, making it a critical biomarker for studying obesity, metabolic disorders, and other related conditions.
Understanding leptin levels can provide valuable insights into the pathophysiology of these diseases and aid in the development of targeted treatments.Overall, the Rat LEP (Leptin) CLIA Kit from Assay Genie is a valuable tool for researchers studying leptin function and its implications in various physiological and pathological processes.
Product Name:
Rat LEP (Leptin) CLIA Kit
SKU:
RTES00359
Target:
Rat LEP (Leptin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat LEP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat LEP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat LEP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat LEP. You can calculate the concentration of Rat LEP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-104
95-108
85-98
Average (%)
96
101
90
1:4
Range (%)
88-99
88-100
88-101
Average (%)
94
93
93
1:8
Range (%)
86-98
90-103
87-100
Average (%)
93
97
92
1:16
Range (%)
101-116
86-101
101-116
Average (%)
107
93
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-107
102
EDTA plasma (n=5)
94-107
100
Cell culture media (n=5)
92-108
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
99.97
293.39
865.85
101.83
275.26
800.74
Standard deviation
9.56
31.57
65.89
10.44
27.17
89.52
C V (%)
9.56
10.76
7.61
10.25
9.87
11.18
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat LEP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat LEP in samples. No significant cross-reactivity or interference between Rat LEP and analogues was observed.
