The Rat MBP (Myelin Basic Protein) CLIA Kit is specially designed for the precise measurement of MBP levels in rat serum, plasma, and cell culture supernatants. This kit is known for its exceptional sensitivity and specificity, ensuring accurate and consistent results for various research purposes.MBP is a key protein found in the myelin sheath surrounding nerve fibers in the central nervous system. Abnormalities in MBP levels have been linked to conditions like multiple sclerosis and other demyelinating disorders, making it a valuable biomarker for studying these diseases and potential therapeutic interventions.
With its reliable performance and broad applicability, the Rat MBP CLIA Kit is a valuable tool for researchers investigating the role of MBP in neurodegenerative disorders and exploring new treatment strategies.
Product Name:
Rat MBP (Myelin Basic Protein) CLIA Kit
SKU:
RTES00397
Target:
Rat MBP (Myelin Basic Protein)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat MBP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat MBP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat MBP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat MBP. You can calculate the concentration of Rat MBP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-105
102-116
103-115
Average (%)
97
107
108
1:4
Range (%)
85-100
98-111
88-98
Average (%)
92
104
93
1:8
Range (%)
84-97
100-116
100-113
Average (%)
91
108
107
1:16
Range (%)
102-116
93-106
96-111
Average (%)
110
100
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-103
98
EDTA plasma (n=5)
92-103
97
Cell culture media (n=5)
95-107
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
106.37
311.9
882.94
97.41
326.7
836.0
Standard deviation
11.13
31.38
57.3
9.65
29.14
59.61
C V (%)
10.46
10.06
6.49
9.91
8.92
7.13
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat MBP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat MBP in samples. No significant cross-reactivity or interference between Rat MBP and analogues was observed.
