Rat MCP-3 (Monocyte Chemotactic Protein 3) CLIA Kit
The Rat MCP-3 (Monocyte Chemotactic Protein-3) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of MCP-3 levels in rat serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a variety of research applications.MCP-3 is a key chemokine involved in recruiting monocytes to sites of inflammation and playing a role in the immune response.
Its levels can be indicative of inflammatory processes and may be associated with conditions such as autoimmune diseases, infections, and cancer. The Rat MCP-3 CLIA Kit is an essential tool for studying these conditions and potential therapeutic interventions.
Product Name:
Rat MCP-3 (Monocyte Chemotactic Protein 3) CLIA Kit
SKU:
RTES00394
Target:
Rat MCP-3 (Monocyte Chemotactic Protein 3)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat MCP-3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat MCP-3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat MCP-3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat MCP-3. You can calculate the concentration of Rat MCP-3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-95
89-101
84-96
Average (%)
90
95
91
1:4
Range (%)
85-100
86-100
87-101
Average (%)
91
93
92
1:8
Range (%)
87-101
88-103
94-105
Average (%)
94
95
100
1:16
Range (%)
92-103
90-101
98-113
Average (%)
97
95
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-111
102
EDTA plasma (n=5)
98-109
104
Cell culture media (n=5)
92-103
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
25.2
46.32
217.94
25.35
48.32
197.35
Standard deviation
2.92
3.41
16.85
3.05
5.37
13.62
C V (%)
11.59
7.36
7.73
12.03
11.11
6.9
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat MCP-3 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat MCP-3 in samples. No significant cross-reactivity or interference between Rat MCP-3 and analogues was observed.
