Description
Rat MCP-3 (Monocyte Chemotactic Protein 3) ELISA Kit
The Rat MCP-3 (Monocyte Chemotactic Protein 3) ELISA Kit is a specialized assay designed for the quantitative analysis of MCP-3 levels in various rat biological samples. MCP-3, also known as CCL7, is a chemokine involved in immune cell recruitment, particularly in the attraction of monocytes and lymphocytes to sites of inflammation.
It regulates the migration and activation of these immune cells, contributing to the body's immune response and inflammatory processes. This ELISA kit offers researchers a reliable method to measure and quantify MCP-3 levels in rat samples. By accurately detecting MCP-3, researchers can gain valuable insights into immune cell behaviors, inflammatory responses, and pathological conditions where MCP-3 plays a role. The Rat MCP-3 ELISA Kit provides high sensitivity and specificity, ensuring robust and reproducible results. With its stringent quality control standards, this kit is optimized for performance and ease of use, making it an excellent choice for research applications focused on immune cell recruitment and inflammation regulation in rat models.
| Product Name: | Rat MCP-3 (Monocyte Chemotactic Protein 3) ELISA Kit |
| Product Code: | AEES00486 |
| Assay Type: | Sandwich |
| Format: | 96T |
| Assay Time: | 3.5h |
| Reactivity: | Rat |
| Detection Range: | 15.63-1000 pg/mL |
| Sensitivity: | 9.38 pg/mL |
| Sample Type & Sample Volume: | Serum, plasma and other biological fluids, 100?L |
| Specificity: | This kit recognizes Rat MCP-3 in samples. No significant cross-reactivity or interference between Rat MCP-3 and analogues was observed. |
| Reproducibility: | Both intra-CV and inter-CV are < 10%. |
| Application: | This ELISA kit applies to the in vitro quantitative determination of Rat MCP-3 concentrations in Serum, plasma and other biological fluids. |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat MCP-3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat MCP-3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat MCP-3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat MCP-3. You can calculate the concentration of Rat MCP-3 in the samples by comparing the OD of the samples to the standard curve.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat MCP-3 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat MCP-3 were tested on 3 different plates, 20 replicates in each plate.
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 47.76 | 101.42 | 379.97 | 50.21 | 97.38 | 398.22 |
| Standard deviation | 2.71 | 5.79 | 12.20 | 3.04 | 5.41 | 12.19 |
| CV (%) | 5.67 | 5.71 | 3.21 | 6.05 | 5.56 | 3.06 |
Recovery
The recovery of Rat MCP-3 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 96-110 | 101 |
| EDTA plasma (n=8) | 93-106 | 100 |
| Cell culture media (n=8) | 93-109 | 100 |
| Kit Components: | An unopened kit can be stored at 2-8? for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
|
| 1. | Add 100?L standard or sample to the wells. Incubate for 90 min at 37°C |
| 2. | Discard the liquid, immediately add 100?L Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
| 3. | Aspirate and wash the plate for 3 times |
| 4. | Add 100?L HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
| 5. | Add 90?L Substrate Reagent. Incubate for 15 min at 37°C |
| 6. | Add 50?L Stop Solution |
| 7. | Read the plate at 450nm immediately. Calculation of the results |
