Rat Monocyte Chemotactic Protein 3 (MCP3) ELISA Kit
The Rat Monocyte Chemotactic Protein 3 (MCP3) ELISA Kit is a powerful tool for the quantitative detection of MCP3 levels in rat samples, including serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.MCP3 is a key chemokine involved in the recruitment and activation of monocytes, playing a critical role in inflammatory responses and immune cell migration.
By measuring MCP3 levels, researchers can gain valuable insights into immune system function, inflammation processes, and potential therapeutic targets for various diseases.With its reliable performance and user-friendly design, the Rat MCP3 ELISA Kit is an indispensable resource for studying the role of MCP3 in health and disease, offering researchers a valuable tool for advancing their research and developing novel treatments.
Product Name:
Rat Monocyte Chemotactic Protein 3 (MCP3) ELISA Kit
Product Code:
RTDL00657
Size:
96 Assays
Target:
MCP3
Synonyms:
CCL7, FIC, MARC, NC28, MCP3, SCYA7, Chemokine C-C-Motif Ligand 7, Small Inducible Cytokine A7, Monocyte Chemoattractant Protein 3
Detection Method:
Sandwich
Range:
15.625-1,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
