The Rat Myo (Myoglobin) CLIA Kit is specifically designed for the precise quantification of myoglobin levels in rat serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and accuracy, ensuring consistent and dependable results for a variety of research purposes.Myoglobin is a key protein found in muscle tissue, acting as an oxygen carrier and aiding in oxygen storage and delivery during muscle activity. Elevated levels of myoglobin are often indicative of muscle damage or injury, making it an important biomarker for studying muscle-related disorders and injuries.
With its high performance and reliability, the Rat Myo (Myoglobin) CLIA Kit is an invaluable tool for researchers studying muscle physiology, exercise science, and various muscle-related pathologies. Its ability to provide precise and reproducible results makes it an essential asset for advancing scientific understanding in the field.
Product Name:
Rat MYO (Myoglobin) CLIA Kit
SKU:
RTES00032
Target:
Rat MYO (Myoglobin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
75.00 pg/mL
Detection range:
125.00-8000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat MYO. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat MYO and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat MYO, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat MYO. You can calculate the concentration of Rat MYO in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-115
85-96
89-99
Average (%)
105
91
94
1:4
Range (%)
101-118
103-121
95-110
Average (%)
108
110
100
1:8
Range (%)
86-97
86-102
103-116
Average (%)
92
93
109
1:16
Range (%)
89-102
92-105
99-112
Average (%)
96
99
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-102
96
EDTA plasma (n=5)
91-104
97
Cell culture media (n=5)
89-105
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
413.34
961.75
3736.54
439.09
903.05
3733.07
Standard deviation
33.77
114.74
311.63
41.54
81.0
345.31
C V (%)
8.17
11.93
8.34
9.46
8.97
9.25
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat MYO concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat MYO in samples. No significant cross-reactivity or interference between Rat MYO and analogues was observed.
