Rat NE/ELA2 (Neutrophil Elastase/Elastase-2) ELISA Kit
The Rat Neutrophil Elastase/Elastase-2 (NE/ELA2) ELISA Kit is a state-of-the-art assay meticulously crafted for the precise quantitative measurement of NE/ELA2 levels in various rat biological samples. Neutrophil elastase, also known as ELA2, is a crucial component of the innate immune response, with essential roles in inflammation, immune regulation, and tissue remodeling. Monitoring NE/ELA2 levels provides valuable insights into neutrophil activation and various pathological conditions associated with inflammatory responses. This cutting-edge ELISA kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results critical for studying neutrophil function and immune-related disorders.
Manufactured under stringent quality control standards, this kit delivers robust performance and user-friendly protocols, making it an indispensable tool for researchers investigating neutrophil biology and immunological responses. Trust the Rat NE/ELA2 ELISA Kit to advance your research in the field of immunology and inflammatory diseases.
Product Name:
Rat NE/ELA2 (Neutrophil Elastase/Elastase-2) ELISA Kit
SKU:
AEES00312
Target:
Rat NE/ELA2 (Neutrophil Elastase/Elastase-2)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat NE/ELA2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat NE/ELA2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat NE/ELA2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat NE/ELA2. You can calculate the concentration of Rat NE/ELA2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-106
100-115
99-115
Average (%)
100
105
105
1:4
Range (%)
93-107
85-99
88-100
Average (%)
98
90
95
1:8
Range (%)
85-100
83-95
83-94
Average (%)
92
89
88
1:16
Range (%)
92-107
82-95
82-93
Average (%)
99
87
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-105
98
EDTA plasma (n=5)
88-98
93
Cell culture media (n=5)
86-99
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
5.47
11.12
48.78
5.6
10.32
53.33
Standard deviation
0.29
0.56
1.53
0.29
0.42
1.75
C V (%)
5.3
5.04
3.14
5.18
4.07
3.28
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat NE/ELA2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat NE/ELA2 in samples. No significant cross-reactivity or interference between Rat NE/ELA2 and analogues was observed.
