Rat NOS2/iNOS (Nitric Oxide Synthase 2, Inducible) CLIA Kit
The Rat NOS2 (iNOS, Nitric Oxide Synthase 2, Inducible) CLIA Kit is specifically designed for the quantitative detection of inducible nitric oxide synthase 2 levels in rat samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reliable results for various research applications.Inducible nitric oxide synthase 2 is an enzyme that plays a crucial role in the production of nitric oxide, a potent signaling molecule involved in various physiological processes such as immune response, inflammation, and vasodilation.
Dysregulation of iNOS has been linked to a wide range of diseases including inflammatory diseases, neurodegenerative disorders, and cancer, making it a valuable target for research and potential therapeutic interventions.With the Rat NOS2 CLIA Kit, researchers can accurately measure iNOS levels in rat samples, providing valuable insights into the role of nitric oxide signaling in health and disease. This kit offers a convenient and efficient solution for studying the mechanisms underlying iNOS regulation and its implications for various pathophysiological conditions.
Product Name:
Rat NOS2/iNOS (Nitric Oxide Synthase 2, Inducible) CLIA Kit
SKU:
RTES00307
Target:
Rat NOS2/iNOS (Nitric Oxide Synthase 2, Inducible)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat NOS2/iNOS. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat NOS2/iNOS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat NOS2/iNOS, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat NOS2/iNOS. You can calculate the concentration of Rat NOS2/iNOS in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-116
91-107
86-100
Average (%)
108
99
92
1:4
Range (%)
89-100
89-100
99-112
Average (%)
95
94
106
1:8
Range (%)
89-101
93-105
93-106
Average (%)
95
98
99
1:16
Range (%)
86-101
93-108
94-108
Average (%)
92
99
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-112
103
EDTA plasma (n=5)
94-105
99
Cell culture media (n=5)
90-102
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
206.0
591.22
1353.96
215.77
535.23
1362.46
Standard deviation
21.53
58.12
138.65
27.36
42.18
106.14
C V (%)
10.45
9.83
10.24
12.68
7.88
7.79
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat NOS2/iNOS concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat NOS2/iNOS in samples. No significant cross-reactivity or interference between Rat NOS2/iNOS and analogues was observed.
