The Rat NRP2 (Neuropilin 2) ELISA Kit is a cutting-edge tool designed to accurately quantify the levels of Neuropilin 2 in various rat biological samples. Neuropilin 2 is a multifunctional type I transmembrane receptor with diverse roles in axonal guidance, angiogenesis, and immune regulation. Its interactions with semaphorins and vascular endothelial growth factors (VEGFs) contribute significantly to neural development, angiogenesis, and immune response modulation. This ELISA kit empowers researchers to precisely measure and analyze the expression of Neuropilin 2, facilitating a deeper understanding of its intricate functions in normal physiological processes and pathological conditions. By elucidating the signaling pathways associated with Neuropilin 2, researchers can explore its involvement in neural development, vascular biology, and immunomodulation, paving the way for potential therapeutic advancements and biomedical applications. The Rat NRP2 ELISA Kit offers remarkable sensitivity and specificity, ensuring accurate and reproducible results in the laboratory. Manufactured with stringent quality control measures, this kit guarantees robust performance and user-friendly protocols, making it an indispensable tool for scientific research aimed at unraveling the complexities of Neuropilin 2 biology in rat models.
Product Name:
Rat NRP2 (Neuropilin 2) ELISA Kit
SKU:
AEES00560
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
