The Rat OC/BGP (Osteocalcin) ELISA Kit is a cutting-edge assay designed for the precise quantitative measurement of Osteocalcin levels in various rat biological samples. Osteocalcin, also known as Bone Gla Protein (BGP), is a key non-collagenous protein found in bone tissue and is essential for bone formation, mineralization, and bone metabolism regulation. This ELISA kit provides researchers with a powerful tool to investigate osteoblast activity, bone turnover, and the role of Osteocalcin in bone health and homeostasis. Accurate assessment of Osteocalcin levels using the Rat OC/BGP ELISA Kit enables researchers to gain valuable insights into bone remodeling processes, bone growth, and the effects of various factors on bone metabolism.
By quantifying Osteocalcin concentrations, researchers can better understand the interplay between bone formation and resorption, leading to advancements in bone-related research, therapy development, and disease mechanisms elucidation. The Rat OC/BGP ELISA Kit by Assay Genie offers exceptional sensitivity and specificity, ensuring accurate and reproducible results. With its stringent quality control measures, this kit delivers robust performance, making it an excellent choice for research projects focused on unraveling the intricate mechanisms underlying bone biology and skeletal health.
Product Name:
Rat OC/BGP (Osteocalcin) ELISA Kit
SKU:
AEES00361
Target:
Rat OC/BGP (Osteocalcin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat OC/BGP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat OC/BGP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat OC/BGP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat OC/BGP. You can calculate the concentration of Rat OC/BGP in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-99
100-115
89-102
Average (%)
91
106
95
1:4
Range (%)
99-113
83-93
94-107
Average (%)
107
88
101
1:8
Range (%)
98-113
83-98
91-107
Average (%)
105
90
99
1:16
Range (%)
101-114
81-93
98-111
Average (%)
107
88
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-98
91
EDTA plasma (n=5)
86-96
91
Cell culture media (n=5)
95-109
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.7
4.7
20.1
2.8
5.1
19.8
Standard deviation
0.1
0.3
1.0
0.2
0.2
0.8
C V (%)
3.7
6.38
4.98
7.14
3.92
4.04
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat OC/BGP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat OC/BGP in samples. No significant cross-reactivity or interference between Rat OC/BGP and analogues was observed.
