The Rat OPG (Osteoprotegerin) ELISA Kit is an advanced assay system that enables precise and quantitative analysis of Osteoprotegerin levels in a wide range of biological samples obtained from rat models. Osteoprotegerin, a key regulatory protein in bone metabolism and cardiovascular health, serves as a critical modulator of bone resorption, vascular calcification, and inflammation. By accurately measuring Osteoprotegerin concentrations, this ELISA kit offers valuable insights into bone homeostasis, vascular health, and inflammatory processes in rat subjects. Accurate quantification of Osteoprotegerin levels is essential for studying the intricate interplay between bone biology, cardiovascular function, and inflammatory responses. The Rat OPG ELISA Kit empowers researchers to explore the role of Osteoprotegerin in bone remodeling, vascular health, and inflammatory diseases, providing a deeper understanding of the underlying mechanisms and potential therapeutic targets. Crafted with a focus on accuracy and reliability, the Rat OPG ELISA Kit delivers exceptional sensitivity and specificity, ensuring consistent and reproducible results. Researchers can rely on this kit to generate reliable data, facilitating in-depth investigations into the physiological and pathological functions of Osteoprotegerin in rat models.
Product Name:
Rat OPG (Osteoprotegerin) ELISA Kit
SKU:
AEES00521
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
