The Rat PCT (Procalcitonin) CLIA Kit is a cutting-edge assay designed for the precise quantification of procalcitonin levels in rat serum, plasma, and tissue homogenates. This innovative kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.Procalcitonin is a key biomarker in the body's response to bacterial infections, sepsis, and inflammatory conditions. Monitoring procalcitonin levels can provide valuable insights into the severity of infections and guide clinical decision-making for optimal patient care.
Whether studying infectious diseases, sepsis, or inflammation, the Rat PCT CLIA Kit is a valuable tool for researchers seeking to better understand these conditions and develop effective therapeutic interventions. Trust in the reliability and precision of this advanced assay kit to elevate your research to new heights.
Product Name:
Rat PCT (Procalcitonin) CLIA Kit
SKU:
RTES00463
Target:
Rat PCT (Procalcitonin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat PCT. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PCT and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PCT, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat PCT. You can calculate the concentration of Rat PCT in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-117
97-113
96-110
Average (%)
109
105
104
1:4
Range (%)
84-98
89-101
95-110
Average (%)
91
94
101
1:8
Range (%)
93-107
101-117
89-103
Average (%)
101
108
96
1:16
Range (%)
94-109
86-100
89-102
Average (%)
100
93
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-112
104
EDTA plasma (n=5)
89-103
96
Cell culture media (n=5)
91-107
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
23.27
46.39
241.81
23.3
45.75
219.34
Standard deviation
2.43
5.26
25.17
1.99
4.41
16.21
C V (%)
10.44
11.34
10.41
8.54
9.64
7.39
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat PCT concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat PCT in samples. No significant cross-reactivity or interference between Rat PCT and analogues was observed.
