Rat PDGF AA Recombinant Protein (RPPB0835)
- SKU:
- RPPB0835
- Product Type:
- Recombinant Protein
- Species:
- Rat
- Uniprot:
- P28576
- Research Area:
- Growth Factors & Cytokines
Description
| Product Name: | Rat PDGF AA Recombinant Protein |
| Product Code: | RPPB0835 |
| Size: | 10µg |
| Species: | Rat |
| Target: | PDGF AA |
| Synonyms: | Platelet-derived growth factor subunit A, PDGF subunit A, PDGF-1, Platelet-derived growth factor A chain, Platelet-derived growth factor alpha polypeptide, Pdgfa, Rpa1. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | PDGF-AA was lyophilized from a 0.2�m filtered concentrated solution in PBS, pH7.0. |
| Solubility: | It is recommended to reconstitute the lyophilized Platelet-derived Growth Factor-AA in Sterile 4mM HCl to a concentration of 0.1-1.0 mg/ml. Further dilutions should be made in appropriate buffered solutions. |
| Stability: | Lyophilized Platelet-derived Growth Factor AA although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PDGF-AA should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
| Purity: | Greater than 97.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Amino Acid Sequence: | MSIEEAIPAV CKTRTVIYEI PRSQVDPTSA NFLIWPPCVE VKRCTGCCNT SSVKCQPSRV HHRSVKVAKV EYVRKKPKLK EVQVRLEEHL ECACATSNLN PDHREEETDV R |
| Biological Activity: | The ED50, as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 5.0 ng/ml, corresponding to a specific activity of > 2.0 � 105 IU/mg. |
PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGF is stored in platelet alpha-granules and released upon platelet activation. The PDGF is involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGF have been identified and named PDGFR-alpha and PDGFR-beta. PDGFR-alpha is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-beta interacts with only PDGF-BB and PDGF-AB.
Platelet-derived Growth Factor AA Human Recombinant is a disulfide-linked homodimer Consists of two A chains containing 111 amino acids each and having a total molecular mass of 25.3KDa. PDGF-AA is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal lung alveolar septum formation during embryogenesis, normal development of the gastrointestinal tract, normal development of Leydig cells and spermatogenesis. Required for normal oligodendrocyte development and normal myelination in the spinal cord and cerebellum. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFB. |
| NCBI Summary: | alpha chain of platelet derived growth factor; acts as a homodimer or heterodimer with PDGF beta to activate PDGF receptors [RGD, Feb 2006] |
| UniProt Code: | P28576 |
| NCBI GenInfo Identifier: | 29789040 |
| NCBI Gene ID: | 25266 |
| NCBI Accession: | NP_036933.1 |
| UniProt Related Accession: | P28576 |
| Molecular Weight: | 22,338 Da |
| NCBI Full Name: | platelet-derived growth factor subunit A |
| NCBI Synonym Full Names: | platelet-derived growth factor alpha polypeptide |
| NCBI Official Symbol: | Pdgfa�� |
| NCBI Official Synonym Symbols: | PDGFACP�� |
| NCBI Protein Information: | platelet-derived growth factor subunit A |
| UniProt Protein Name: | Platelet-derived growth factor subunit A |
| UniProt Synonym Protein Names: | PDGF-1; Platelet-derived growth factor A chain; Platelet-derived growth factor alpha polypeptide |
| Protein Family: | Platelet-derived growth factor |
| UniProt Gene Name: | Pdgfa�� |
| UniProt Entry Name: | PDGFA_RAT |
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