Rat PDGF-D (Platelet Derived Growth Factor D) CLIA Kit
The Rat PDGF-D (Platelet-Derived Growth Factor D) CLIA Kit is specifically designed for the accurate detection of PDGF-D levels in rat serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.PDGF-D is an important growth factor that plays a key role in cell growth, proliferation, and differentiation. It is known to be involved in processes such as wound healing, tissue repair, and angiogenesis. Dysregulation of PDGF-D has been linked to various diseases including cancer, fibrosis, and cardiovascular disorders, making it a valuable biomarker for studying these conditions and potential therapeutic targets.
With the Rat PDGF-D CLIA Kit, researchers can accurately measure PDGF-D levels in rat samples, providing valuable insights into biological pathways and disease mechanisms. This kit is a valuable tool for understanding the role of PDGF-D in physiological and pathological processes, as well as for drug discovery and development efforts.
Product Name:
Rat PDGF-D (Platelet Derived Growth Factor D) CLIA Kit
SKU:
RTES00451
Target:
Rat PDGF-D (Platelet Derived Growth Factor D)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat PDGF-D. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PDGF-D and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PDGF-D, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat PDGF-D. You can calculate the concentration of Rat PDGF-D in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-102
100-115
97-113
Average (%)
96
107
103
1:4
Range (%)
97-113
95-109
101-117
Average (%)
105
101
107
1:8
Range (%)
87-99
94-109
93-105
Average (%)
94
101
100
1:16
Range (%)
96-110
87-102
84-96
Average (%)
102
94
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-108
100
EDTA plasma (n=5)
99-114
107
Cell culture media (n=5)
87-100
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
221.61
340.27
1365.76
229.88
341.6
1264.12
Standard deviation
17.97
31.54
153.92
24.64
39.15
88.24
C V (%)
8.11
9.27
11.27
10.72
11.46
6.98
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat PDGF-D concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat PDGF-D in samples. No significant cross-reactivity or interference between Rat PDGF-D and analogues was observed.
