Rat P1NP (Procollagen Type I N-Terminal Propeptide) ELISA Kit
The Rat PINP (Procollagen Type I N-Terminal Propeptide) Elisa Kit is a reliable and accurate tool for detecting levels of PINP in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, providing researchers with robust and dependable results for a variety of research applications.PINP is an important protein that plays a key role in collagen synthesis and bone formation. Monitoring PINP levels can provide valuable insights into bone metabolism, bone health, and conditions such as osteoporosis.
This ELISA kit enables researchers to study the role of PINP in various physiological and pathological processes, ultimately aiding in the development of new therapeutic strategies for bone-related disorders.Overall, the Rat PINP Elisa Kit is a valuable tool for studying bone metabolism and bone health in preclinical research settings, offering researchers a reliable method for quantifying PINP levels in rat samples with high precision and accuracy.
Product Name:
Rat P1NP (Procollagen Type I N-Terminal Propeptide) ELISA Kit
SKU:
RTES00990
Target:
Rat P1NP (Procollagen Type I N-Terminal Propeptide)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat Pâ… NP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat Pâ… NP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat Pâ… NP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat Pâ… NP. You can calculate the concentration of Rat Pâ… NP in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-103
88-101
95-108
Average (%)
96
94
102
1:4
Range (%)
89-103
81-96
85-96
Average (%)
96
88
90
1:8
Range (%)
88-100
86-99
88-103
Average (%)
95
92
94
1:16
Range (%)
88-101
83-98
85-99
Average (%)
93
89
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-99
90
EDTA plasma (n=5)
89-103
95
Cell culture media (n=5)
89-102
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
211.34
396.29
1351.6
208.17
360.15
1320.24
Standard deviation
12.98
17.24
62.04
12.28
15.41
60.73
C V (%)
6.14
4.35
4.59
5.9
4.28
4.6
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat Pâ… NP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat Pâ… NP in samples. No significant cross-reactivity or interference between Rat Pâ… NP and analogues was observed.
