The Rat Prothyroliberin (TRH) ELISA Kit is a powerful tool for measuring levels of prothyroliberin (TRH) in rat samples such as serum, plasma, and cell culture supernatants. This kit is designed with high sensitivity and specificity, ensuring accurate and reliable results for a variety of research applications. Prothyroliberin, also known as thyrotropin-releasing hormone (TRH), is a key neuropeptide involved in regulating thyroid function and neuroendocrine pathways.
Dysregulation of TRH has been implicated in disorders such as thyroid dysfunction and mood disorders, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions. With the Rat Prothyroliberin (TRH) ELISA Kit, researchers can gain valuable insights into the role of TRH in various physiological and pathological processes, advancing our understanding of thyroid function and related diseases.
Product Name:
Rat Prothyroliberin/Trh ELISA Kit
SKU:
RTFI00255
Reactivity:
Rat
Assay Type:
Competitive ELISA, Coated with Antigen
Sensitivity:
0.094 uIU/mL
Range:
0.156-10 uIU/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C(Sealed), Don't cryopreserve.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 10)
89-100%
85-102%
88-105%
EDTA Plasma (n = 10)
85-101%
82-94%
93-100%
Heparin Plasma (n = 10)
80-93%
82-95%
84-98%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 10)
93-100
95
EDTA Plasma (n = 10)
86-101
83
Heparin Plasma (n = 10)
81-98
96
Note: The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate TMB substrate for 30 minutes at room temperature. Prepare standards, samples (minimum 1:2 dilution), blanks, assign wells, and pre-wash the plate twice.
2
Sample & Biotin-Antibody Binding: Add 50 µL standard or sample followed by 50 µL biotin-labeled antibody to each well. Mix gently and incubate at 37°C for 45 minutes.
3
Washing: Wash the plate 5 times with wash buffer, allowing 1 minute soak time per wash.
4
Color Development: Add TMB substrate and incubate in the dark at 37°C for 10-20 minutes until color develops.
5
Stop Reaction: Add stop solution to terminate the reaction. The color changes from blue to yellow immediately.
6
Reading: Measure absorbance at 450 nm using a microplate reader.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant and store appropriately.
Plasma
Collect using EDTA anticoagulant, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Cell Culture Supernatant
Centrifuge at 1000 × g for 20 minutes at 4°C and collect clarified supernatant.
Cell Lysate
Lyse cells using recommended lysis buffer with protease inhibitors, centrifuge at 10,000 rpm for 10 minutes, and collect protein supernatant.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge at 5000 × g for 5 minutes, and collect supernatant.
Other Sample Types
Centrifuge samples at 1000 × g for 15 minutes at 2–8°C and collect supernatant. For additional guidance, please contact techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
