The Rat PS1 (Presenilin-1) CLIA Kit is a specialized assay designed for the quantitative measurement of Presenilin-1 levels in rat samples. This kit offers high sensitivity and accuracy, allowing for reliable and reproducible results in a variety of research settings.Presenilin-1 is a key protein involved in the processing of amyloid precursor protein and the production of beta-amyloid peptides, which are closely linked to the development of Alzheimer's disease. By measuring Presenilin-1 levels, researchers can gain valuable insights into the pathogenesis of neurodegenerative disorders and potentially identify new therapeutic targets.
The Rat PS1 CLIA Kit is easy to use and provides rapid results, making it a valuable tool for studying the role of Presenilin-1 in disease progression and exploring novel treatment strategies. With its high performance and versatility, this kit is ideal for researchers looking to advance their understanding of Alzheimer's disease and other related conditions.
Product Name:
Rat PS1 (Presenilin 1) CLIA Kit
SKU:
RTES00629
Target:
Rat PS1 (Presenilin 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat PS1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PS1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PS1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat PS1. You can calculate the concentration of Rat PS1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-114
97-111
91-106
Average (%)
105
104
99
1:4
Range (%)
87-101
87-103
92-106
Average (%)
94
94
99
1:8
Range (%)
95-111
97-111
95-108
Average (%)
103
103
102
1:16
Range (%)
97-111
100-115
94-109
Average (%)
104
106
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-101
95
EDTA plasma (n=5)
95-112
102
Cell culture media (n=5)
96-111
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
102.96
256.54
990.62
105.84
246.68
922.03
Standard deviation
9.2
23.24
61.22
12.79
25.46
73.58
C V (%)
8.94
9.06
6.18
12.08
10.32
7.98
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat PS1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat PS1 in samples. No significant cross-reactivity or interference between Rat PS1 and analogues was observed.
