The Rat PSA (Prostate Specific Antigen) CLIA Kit is specifically designed for the precise measurement of PSA levels in rat serum, plasma, and cell culture supernatants. This kit offers superior sensitivity and specificity, guaranteeing consistent and accurate results for various research purposes.PSA is a key protein associated with prostate health, serving as a biomarker for prostate cancer and other prostate-related conditions.
By detecting PSA levels, researchers can gain valuable insights into the progression of these diseases and assess treatment outcomes.With its reliable performance and versatility, the Rat PSA CLIA Kit is an invaluable tool for investigating prostate health and developing innovative therapeutic interventions. Order now to advance your research with confidence and precision.
Product Name:
Rat PSA (Prostate Specific Antigen) CLIA Kit
SKU:
RTES00480
Target:
Rat PSA (Prostate Specific Antigen)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat PSA. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PSA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PSA, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat PSA. You can calculate the concentration of Rat PSA in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-107
97-114
94-112
Average (%)
100
104
102
1:4
Range (%)
102-120
90-103
88-103
Average (%)
109
95
94
1:8
Range (%)
100-111
88-101
92-108
Average (%)
106
94
99
1:16
Range (%)
100-119
89-103
91-105
Average (%)
108
95
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-116
106
EDTA plasma (n=5)
88-100
94
Cell culture media (n=5)
92-106
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
91.61
296.52
908.4
92.87
292.1
829.66
Standard deviation
11.52
32.85
100.65
9.78
34.03
77.99
C V (%)
12.58
11.08
11.08
10.53
11.65
9.4
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat PSA concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat PSA in samples. No significant cross-reactivity or interference between Rat PSA and analogues was observed.
