Rat Retinol Binding Protein 4, Plasma (RBP4) ELISA Kit
The Rat Retinol-Binding Protein 4 (RBP4) Plasma ELISA Kit is a highly sensitive and specific assay designed for the accurate quantification of RBP4 levels in rat plasma samples. This kit is ideal for researchers studying metabolic disorders, diabetes, and cardiovascular diseases where RBP4 plays a crucial role as a biomarker.RBP4 is a carrier protein for retinol and is involved in the regulation of insulin resistance and glucose metabolism. Elevated levels of RBP4 have been linked to insulin resistance, obesity, and other metabolic disorders, making it a valuable target for research and potential therapeutic developments.
With its high performance and reliability, the Rat RBP4 Plasma ELISA Kit offers researchers a powerful tool for investigating the role of RBP4 in various physiological and pathological conditions, providing valuable insights for future studies and clinical applications.
Product Name:
Rat Retinol Binding Protein 4, Plasma (RBP4) ELISA Kit
Product Code:
RTDL00898
Size:
96 Assays
Target:
RBP4
Synonyms:
PRBP, RBP, Plasma retinol-binding protein
Detection Method:
Sandwich
Range:
1.56-100ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
