The Rat SHBG (Sex Hormone Binding Globulin) CLIA Kit is a reliable and sensitive assay designed for the precise measurement of SHBG levels in rat serum, plasma, and cell culture supernatants. This kit provides accurate and reproducible results, making it ideal for a variety of research applications.SHBG is a key protein that binds to sex hormones, regulating their bioavailability and activity in the body. It plays a crucial role in hormone balance and various physiological processes, making it an important biomarker for studying hormonal disorders, reproductive health, and metabolic conditions.
With its high sensitivity and specificity, the Rat SHBG CLIA Kit is a valuable tool for researchers investigating the role of sex hormones in health and disease, offering insights into potential therapeutic interventions and personalized medicine approaches.
Product Name:
Rat SHBG (Sex Hormone-Binding Globulin) CLIA Kit
SKU:
RTES00514
Target:
Rat SHBG (Sex Hormone-Binding Globulin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat SHBG. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat SHBG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat SHBG, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat SHBG. You can calculate the concentration of Rat SHBG in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-105
101-117
87-102
Average (%)
99
107
93
1:4
Range (%)
86-98
94-108
90-106
Average (%)
91
99
97
1:8
Range (%)
86-101
88-102
90-104
Average (%)
92
93
95
1:16
Range (%)
95-109
97-114
83-96
Average (%)
102
104
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-105
97
EDTA plasma (n=5)
98-113
106
Cell culture media (n=5)
97-110
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
27.33
56.19
182.2
26.65
53.92
165.29
Standard deviation
3.32
3.97
11.02
2.72
4.23
18.38
C V (%)
12.15
7.07
6.05
10.21
7.84
11.12
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat SHBG concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat SHBG in samples. No significant cross-reactivity or interference between Rat SHBG and analogues was observed.
