Rat SOD1 (Superoxide Dismutase 1, Soluble) ELISA Kit
The Rat SOD1 (Superoxide Dismutase 1) Soluble ELISA Kit is specifically designed for the accurate quantification of soluble SOD1 levels in rat serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.SOD1 is a critical enzyme that plays a key role in antioxidant defense by catalyzing the dismutation of superoxide radicals. Dysregulation of SOD1 has been implicated in various diseases including neurodegenerative disorders, cardiovascular diseases, and inflammatory conditions. This ELISA kit provides a valuable tool for studying the role of SOD1 in these diseases and developing potential therapeutic interventions.Overall, the Rat SOD1 Soluble ELISA Kit is a reliable and efficient tool for researchers looking to investigate the involvement of SOD1 in various physiological and pathological processes.
Product Name:
Rat SOD1 (Superoxide Dismutase 1, Soluble) ELISA Kit
SKU:
RTES00994
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
