The Rat Sorbitol Dehydrogenase (SDH) ELISA Kit is a powerful tool for the accurate quantification of sorbitol dehydrogenase levels in rat samples. This kit offers high sensitivity and specificity, allowing for precise and consistent results, making it perfect for various research purposes.Sorbitol dehydrogenase is an important enzyme involved in the metabolism of sorbitol, a sugar alcohol commonly found in various tissues. Changes in sorbitol dehydrogenase levels have been linked to conditions such as diabetes, liver disease, and cataracts, highlighting its importance as a potential biomarker for these diseases.
By utilizing the Rat Sorbitol Dehydrogenase (SDH) ELISA Kit, researchers can study the role of sorbitol dehydrogenase in disease pathology and potentially develop new therapeutic strategies targeting this enzyme. With its reliable performance and broad applicability, this kit is a valuable asset for any laboratory conducting research on sorbitol metabolism and related diseases.
Product Name:
Rat Sorbitol Dehydrogenase (SDH) ELISA Kit
Product Code:
RTDL00935
Size:
96 Assays
Target:
SDH
Synonyms:
SORD, L-iditol 2-dehydrogenase
Detection Method:
Sandwich
Range:
1.56-100ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
