The Rat TAT (Thrombin-Antithrombin) Complex CLIA Kit is a highly sensitive and specific assay designed for the quantification of TAT levels in rat serum, plasma, and cell culture supernatants. This kit provides accurate and reproducible results, making it ideal for various research applications.TAT is a key marker of coagulation and thrombosis, representing the formation and inhibition of thrombin in the blood. Monitoring TAT levels can provide valuable insights into the coagulation cascade and pathological conditions related to thrombus formation, such as sepsis, disseminated intravascular coagulation (DIC), and venous thromboembolism.
By utilizing the Rat TAT Complex CLIA Kit, researchers can better understand the mechanisms underlying these conditions and potentially identify new therapeutic targets for intervention. This kit offers a convenient and reliable solution for studying coagulation disorders in rats, contributing to advancements in the field of hemostasis research.
Product Name:
Rat TAT (Thrombin-Antithrombin Complex) CLIA Kit
SKU:
RTES00545
Target:
Rat TAT (Thrombin-Antithrombin Complex)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat TAT. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TAT and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TAT, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat TAT. You can calculate the concentration of Rat TAT in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-103
91-102
92-102
Average (%)
96
97
97
1:4
Range (%)
88-100
94-108
93-108
Average (%)
93
101
99
1:8
Range (%)
88-101
99-115
101-115
Average (%)
94
105
107
1:16
Range (%)
86-99
95-110
92-106
Average (%)
91
102
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-105
96
EDTA plasma (n=5)
100-117
107
Cell culture media (n=5)
90-103
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
209.06
545.43
1341.66
196.47
567.36
1329.34
Standard deviation
24.15
42.0
100.89
23.99
45.22
128.15
C V (%)
11.55
7.7
7.52
12.21
7.97
9.64
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat TAT concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat TAT in samples. No significant cross-reactivity or interference between Rat TAT and analogues was observed.
