Rat betaTG/PBP/CXCL7/NAP2 (Thromboglobulin, Beta) ELISA Kit
The Rat βTG/PBP/CXCL7/NAP2 (Thromboglobulin, Beta) ELISA Kit is a specialized assay developed for the quantitative measurement of β-thromboglobulin (βTG), also known as platelet basic protein (PBP) or neutrophil-activating protein 2 (NAP-2), levels in a variety of rat biological samples. βTG is a chemokine and growth factor stored in the α-granules of platelets that plays a crucial role in platelet activation, chemotaxis of neutrophils, and regulating inflammatory responses. This ELISA kit provides researchers with a precise and reliable method to quantify βTG levels, enabling a deeper understanding of its involvement in platelet activation, inflammation, and cellular signaling pathways. By accurately measuring βTG concentrations, researchers can gain valuable insights into the intricate interplay between platelet function, immune responses, and inflammatory processes, shedding light on the role of βTG in various physiological and pathological conditions. With exceptional sensitivity and specificity, the Rat βTG/PBP/CXCL7/NAP2 ELISA Kit ensures reproducible and accurate results, allowing researchers to explore the biological functions and regulatory mechanisms of βTG with precision. Manufactured following stringent quality control standards, this kit guarantees robust performance and user-friendly operation, making it an excellent choice for researchers investigating the complex roles of βTG in platelet biology, inflammation, and immune modulation.
Product Name:
Rat betaTG/PBP/CXCL7/NAP2 (Thromboglobulin, Beta) ELISA Kit
SKU:
AEES00556
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
96T/48T/24T: 3 vials, 20 mL | 96T*5: 15 vials, 20 mL
2–8°C, 12 months
Biotinylated Detection Ab Diluent
96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL
2–8°C, 12 months
HRP Conjugate Diluent
96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL
2–8°C, 12 months
Concentrated Wash Buffer(25×)
96T/48T/24T: 1 vial, 30 mL | 96T*5: 5 vials, 30 mL
2–8°C, 12 months
Substrate Reagent
96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL
2–8°C (Protect from light)
Stop Solution
96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL
2–8°C
Plate Sealer
96T/48T/24T: 5 pieces | 96T*5: 25 pieces
2–8°C
Technical Manual
1 copy
-
Certificate of Analysis
1 copy
-
Linearity:
Serum (n=5)
EDTA Plasma (n=5)
Cell Culture Media (n=5)
1:2
Range (%)
90-100
88-100
91-108
Average (%)
95
94
98
1:4
Range (%)
90-105
88-102
88-100
Average (%)
96
94
93
1:8
Range (%)
91-105
85-99
88-104
Average (%)
98
91
95
1:16
Range (%)
89-101
85-98
88-102
Average (%)
96
90
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
EDTA Plasma (n=8)
87-99
92
Cell Culture Media (n=8)
83-95
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
50.18
135.87
411.77
52.32
124.47
410.14
Standard deviation
2.63
7.54
25.94
3.3
10.73
26.21
C V (%)
5.24
5.55
6.3
6.3
8.62
6.39
Uniport ID:
Q99ME0
Sample type & Sample volume:
Serum, Plasma And Other Biological Fluids; 100 μL
Specificity:
This kit recognizes Rat βTG/PBP/CXCL7/NAP2 in samples.No significant cross-reactivity or interference between Rat βTG/PBP/CXCL7/NAP2 and analogues was observed
