Rat TIMP-4 (Tissue Inhibitors of Metalloproteinase 4) CLIA Kit
The Rat TIMP-4 (Tissue Inhibitors of Metalloproteinase-4) CLIA Kit from Assay Genie is a powerful tool for measuring TIMP-4 levels in rat tissue samples. This innovative kit offers high sensitivity and specificity, allowing for accurate and reliable results in a variety of research applications.TIMP-4 is a key regulator of metalloproteinases, enzymes that play a critical role in tissue remodeling, cell migration, and inflammation. Dysregulation of TIMP-4 has been implicated in various diseases, including cancer, arthritis, and cardiovascular disorders.
By accurately measuring TIMP-4 levels, researchers can gain valuable insights into disease mechanisms and potential therapeutic targets.The Rat TIMP-4 CLIA Kit is easy to use and provides quick and efficient results, making it an essential tool for any laboratory studying TIMP-4 biology. Trust Assay Genie for high-quality research tools that deliver reliable and reproducible data.
Product Name:
Rat TIMP-4 (Tissue Inhibitors of Metalloproteinase 4) CLIA Kit
SKU:
RTES00559
Target:
Rat TIMP-4 (Tissue Inhibitors of Metalloproteinase 4)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat TIMP-4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TIMP-4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TIMP-4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat TIMP-4. You can calculate the concentration of Rat TIMP-4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-100
100-114
89-101
Average (%)
92
106
94
1:4
Range (%)
95-112
97-113
95-108
Average (%)
102
104
101
1:8
Range (%)
86-100
96-112
98-115
Average (%)
93
103
105
1:16
Range (%)
99-117
90-105
94-112
Average (%)
107
97
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-99
92
EDTA plasma (n=5)
86-101
93
Cell culture media (n=5)
86-98
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
98.36
207.4
993.36
91.92
222.05
945.01
Standard deviation
10.05
19.95
106.79
8.43
16.7
92.61
C V (%)
10.22
9.62
10.75
9.17
7.52
9.8
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat TIMP-4 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat TIMP-4 in samples. No significant cross-reactivity or interference between Rat TIMP-4 and analogues was observed.
