Rat TNF- alpha (Tumor Necrosis Factor Alpha) CLIA Kit
The Rat TNF-Alpha (Tumor Necrosis Factor Alpha) CLIA Kit is specifically designed for the accurate measurement of TNF-Alpha levels in rat samples including serum, plasma, and tissue culture supernatants. This kit provides high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.TNF-Alpha is a key cytokine involved in inflammation, immune response, and cell death. Its dysregulation has been implicated in various diseases including autoimmune disorders, inflammatory conditions, and cancer.
The ability to accurately measure TNF-Alpha levels is crucial for understanding disease mechanisms and developing targeted therapies.The Rat TNF-Alpha CLIA Kit from Assay Genie is a valuable tool for researchers studying the role of TNF-Alpha in disease processes and exploring potential therapeutic interventions. With its high performance and ease of use, this kit is an essential addition to any laboratory conducting research in the field of immunology and inflammation.
Product Name:
Rat TNF- alpha (Tumor Necrosis Factor Alpha) CLIA Kit
SKU:
RTES00017
Target:
Rat TNF- alpha (Tumor Necrosis Factor Alpha)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat TNF-α. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TNF-α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TNF-α, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat TNF-α. You can calculate the concentration of Rat TNF-α in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-101
100-116
102-120
Average (%)
94
106
109
1:4
Range (%)
84-97
101-113
91-104
Average (%)
91
107
98
1:8
Range (%)
85-97
98-113
92-107
Average (%)
91
105
98
1:16
Range (%)
91-103
87-98
86-99
Average (%)
98
92
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-101
92
EDTA plasma (n=5)
99-115
105
Cell culture media (n=5)
98-115
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
53.29
136.93
449.48
49.99
147.84
445.02
Standard deviation
4.73
12.95
37.13
6.23
16.57
29.24
C V (%)
8.88
9.46
8.26
12.46
11.21
6.57
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat TNF-α concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat TNF-α in samples. No significant cross-reactivity or interference between Rat TNF-α and analogues was observed.
