The Rat TRF (Transferrin) CLIA Kit is a highly sensitive assay kit designed for the accurate detection of transferrin levels in rat serum, plasma, and cell culture supernatants. This kit offers high specificity and precision, ensuring consistent and reliable results for various research studies.Transferrin is a key protein involved in iron transport and regulation, playing a crucial role in maintaining iron homeostasis in the body. Abnormalities in transferrin levels have been linked to various conditions such as iron-deficiency anemia, liver diseases, and inflammatory disorders, making it an important biomarker for research and diagnostic purposes.
With its user-friendly format and efficient performance, the Rat TRF (Transferrin) CLIA Kit is an invaluable tool for researchers studying iron metabolism, transferrin biology, and related diseases in rat models. Trust in the accuracy and precision of this kit to advance your research goals and contribute to scientific advancements in the field.
Product Name:
Rat TRF (Transferrin) CLIA Kit
SKU:
RTES00607
Target:
Rat TRF (Transferrin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat TRF. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TRF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TRF, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat TRF. You can calculate the concentration of Rat TRF in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-111
90-104
87-102
Average (%)
103
95
94
1:4
Range (%)
92-104
95-110
85-99
Average (%)
99
103
91
1:8
Range (%)
87-101
87-101
88-103
Average (%)
94
93
95
1:16
Range (%)
98-112
99-117
97-109
Average (%)
106
107
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-102
96
EDTA plasma (n=5)
101-112
106
Cell culture media (n=5)
84-96
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.07
2.64
9.72
1.16
2.62
9.05
Standard deviation
0.09
0.25
0.74
0.11
0.23
1.0
C V (%)
8.41
9.47
7.61
9.48
8.78
11.05
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat TRF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat TRF in samples. No significant cross-reactivity or interference between Rat TRF and analogues was observed.
