The Rat VWF (Von Willebrand Factor) ELISA Kit is a powerful tool for researchers looking to accurately measure levels of VWF in rat serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.Von Willebrand Factor is a key player in the blood clotting process, playing a crucial role in hemostasis and thrombosis. Dysregulation of VWF levels can lead to serious health consequences, making it a valuable biomarker for studying conditions such as von Willebrand disease and other clotting disorders.With the Rat VWF ELISA Kit, researchers can confidently investigate the role of VWF in various physiological and pathological processes, paving the way for potential therapeutic interventions and advancements in the field of hemostasis research.
Product Name:
Rat VWF (Von Willebrand Factor) ELISA Kit
SKU:
RTES00889
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
