The Recombinant Cynomolgus CD3 epsilon/CD3E Protein is a high-quality recombinant protein produced for advanced research applications in molecular biology and biotechnology. This protein serves as a critical reagent in various experimental contexts, including functional studies, binding assays, and therapeutic development programs, providing researchers with a standardized and reliable tool for investigating protein function and interactions.
This product (SKU: RPCB1307) is produced using HEK293 cells and features a C-His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 11.7 kDa with an observed molecular weight of 14-16 kDa under denaturing conditions, achieving ≥ 95 % as determined by Tris-Bis PAGE.≥ 95 % as determined by HPLC.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
High quality, high purity and low endotoxin recombinant Recombinant Cynomolgus CD3 epsilon/CD3E Protein (RPCB1307), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 1 EU/μg of the protein by LAL method.
Purity:
≥ 95 % as determined by Tris-Bis PAGE.≥ 95 % as determined by HPLC.
Formulation:
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
Bio-Activity:
Immobilized Cyno CD3 epsilon at 1 μg/mL(100 μL/Well).Dose response curve for Anti-CD3 Ab. with the EC 50 of 0.2 μg/mL determined by ELISA.
Reconstitution:
Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
CD3E, is a single-pass type I membrane protein.CD3 (cluster of differentiation 3) T cell co-receptor helps to activate both the cytotoxic T cell (CD8+ naive T cells) and also T helper cells (CD4+ naive T cells). It consists of a protein complex and is composed of four distinct chains. In mammals, the complex contains a CD3γ chain, a CD3δ chain, and two CD3ε chains.
Recombinant Cynomolgus CD3 epsilon/CD3E Protein was determined by Tris-Bis PAGE under reducing conditions.
The purity of Cynomolgus CD3E is greater than 95% as determined by SEC-HPLC.
