Recombinant Human Brain Natriuretic Peptide/NT-proBNP Protein
The Recombinant Human Brain Natriuretic Peptide/NT-proBNP Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating Brain Natriuretic Peptide/NT-proBNP function and its implications in health and disease.
This product (SKU: RPCB0709) is produced using HEK293 cells and features a C-hFC tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 34.60 kDa with an observed molecular weight of 35-60 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE., ensuring exceptional quality and consistency for research applications.
Key Features
High Purity by Affinity Chromatography
Mammalian & Bacterial Expression Systems
High lot-to-lot consistency via strict QC
Product Name:
Recombinant Human Brain Natriuretic Peptide/NT-proBNP Protein
High quality, high purity and low endotoxin recombinant Recombinant Human Brain Natriuretic Peptide/NT-proBNP Protein (RPCB0709), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 0.01 EU/μg of the protein by LAL method
Purity:
≥ 95 % as determined by SDS-PAGE.
Formulation:
Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.Contact us for customized product form or formulation.
Reconstitution:
Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Brain-type Natriuretic Peptide (BNP) is a nonglycosylated peptide that is produced predominantly byventricular myocytes and belongs to the natriuretic peptide family. Proteolytic cleavage of the 12 kDa BNPprecursor gives rise to N-terminal Pro BNP (NT-proBNP) and mature BNP. N-terminal proB-type natriureticpeptide (NT-proBNP), a useful marker of heart failure (HF), is considered to be secreted mainly from theventricle, increased serum NT-proBNP levels are also encountered in conditions such as atrial fibrillation (AF)and atrial septal defect in patients without HF.
Recombinant Human Brain Natriuretic Peptide/NT-proBNP Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
