Cathepsin Z (CTSZ); also known as Cathepsin X or CATX; belongs to the C1 family of lysosomal cysteine proteases. Its gene structure and activity properties show several unique features that distinguish it clearly from other human cysteine proteases. It has a very short pro-region that shows no similarity to those of other cathepsins and a three-residue insertion motif that forms a characteristic ‘mini loop’. Cathepsin Z exhibits mono- and di-peptidase activity at its C-terminus; and in contrast to cathepsin B; it does not act as an endopeptidase. It is restricted to the cells of theimmune system; predominantly monocytes; macrophages and dendritic cells. Cathepsin Z is widely expressed in human tissues; suggesting that this enzyme could be involved in the normal intracellular protein degradation taking place in all cell types. It is capable to cleave regulatory motifs at C-terminus affecting the function of targeted molecules. Cathepsin X may regulate also the maturation of dendritic cells; a process; which is crucial in the initiation of adaptive immunity. Furthermore; higher levels of Cathepsin Z are also found in tumour and immune cells of prostate and gastric carcinomas and inmacrophages of gastric mucosa; especially after infection by Helicobacter pylori. Cathepsin Z is also ubiquitously distributed in cancer cell lines and in primary tumors from different sources; suggesting that this enzyme may participate in tumor progression.
Product Name:
Human Cathepsin Z Recombinant Protein (RPES1271)
Product Code:
RPES1271
Size:
10µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
Cathepsin Z, Cathepsin P, Cathepsin X, CTSZ
Accession:
Q9UBR2
Sequence:
Met 1-Val 303
Fusion tag:
C-His
Activity:
Measured by its ability to cleave the fluorogenic peptide substrate; Mca-RPPGFSAFK (Dnp)-OH;R&D Systems; Catalog # ES005.The specific activity is >800 pmoles/min/µg.
Endotoxin:
<1.0 EU per µg of the protein as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the human CTSZ (Q9UBR2) (Met 1-Val 303) was fused with a polyhistidine tag at the C-terminus.
Purity:
> 97 % as determined by reducing SDS-PAGE.
Mol Mass:
33 kDa
Formulation:
Lyophilized from sterile PBS; pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
