CD47(Integrin-Associated Protein;IAP) is a 40 - 60 kDa variably glycosylated atypical member of the immunoglobulin superfamily. The ubiquitously expressed CD47 binds to SIRP family members on macrophages; neutrophils; and T cells. CD47 is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The protein is also a receptor for the C-terminal cell-binding domain of thrombospondin; and it may play a role in membrane transport and signal transduction. This protein has broad tissue distribution; and is reduced in expression on Rh erythrocytes.
Product Name:
Human CD47/IAP Recombinant Protein (RPES1160)
Product Code:
RPES1160
Size:
10µg
Species:
Human
Expressed Host:
Human Cells
Synonyms:
Leukocyte Surface Antigen CD47, Antigenic Surface Determinant Protein OA3, Integrin-Associated Protein, IAP, Protein MER6, CD47, MER6
Accession:
Q08722
Sequence:
Gln19-Pro139
Fusion tag:
C-His
Activity:
Immobilized Human SIRPA-Fc(Cat: PKSH033061) at 10µg/ml(100 ?l/well) can bind Human CD47-His. The ED50 of Human CD47-His is 4.18 ng/ml .
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human CD47 is produced by our Mammalian expression system and the target gene encoding Gln19-Pro139 is expressed with a 6His tag at the C-terminus.
Purity:
> 95% as determined by reducing SDS-PAGE.
Mol Mass:
14.76 kDa
AP Mol Mass:
34 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 10mM Tris-Citrate; 150mM NaCl; pH 8.0.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
