Fibroblast growth factor 2(FGF2) is a secreted protein and belongs to the heparin-binding growth factors family. Epithelium, tumor and other cell types all create FGF2. FGF2 involved in developmental processes and regulates differentiation; proliferation; and migration. FGF2 is a critical factor for growing embryonic stem cells in culture without inducing differentiation. FGF-2 binds heparan sulfate with high affinity, and it is a component of the FGF basic activation of FGFR tyrosine kinase. Human FGF-2/FGFb Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Measured in a cell proliferation assay using Balb/3T3 mouse fibroblast cells. The ED50 for this effect is 0.1-0.6 ng/ml
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Fibroblast growth factor 2/Fibroblast Growth Factor Basic is produced by our E.coli expression system and the target gene encoding Met134-Ser288 is expressed.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
17.2 kDa
AP Mol Mass:
17 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM TrisHCl; 150mM NaCl; pH 7.5.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
