FGF-basic is a members of the Fibroblast Growth Factors (FGFs) family. The FGFs family includes a variety of proteins that play numerous roles in development, such as cell growth and proliferation. The fibroblast growth factor (FGF) family of proteins regulate a wide range of biological activities, including angiogenesis, cell proliferation, pattern formation, embryonic development, metabolic regulation, cellular migration, neurotrophic effects, and tissue healing. As a non-glycosylated heparin binding growth factor, FGF-basic is found in the brain, pituitary, kidney, retina, bone, testis, adrenal gland liver, monocytes, epithelial cells and endothelial cells. FGF-basic signals through FGFR 1b; 1c; 2c; 3c and 4. Human FGF0/FGF10 Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Measured in a cell proliferation assay using BALB/c 3T3 cells. The ED50 for this effect is 0.3-2.0 ng/ml.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Fibroblast growth factor 2/Fibroblast Growth Factor Basic is produced by our E.coli expression system and the target gene encoding Pro143-Ser288 is expressed.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
16.3 kDa
AP Mol Mass:
17 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of PBS; pH7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
