The Recombinant Human Flt3 ligand/Flt3L Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating Flt3 ligand/Flt3L function and its implications in health and disease.
This product (SKU: RPCB1891) is produced using HEK293 cells and features a C-hFc tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 44.00 kDa with an observed molecular weight of 50-65 kDa under denaturing conditions, achieving ≥ 95 % as determined by Tris-Bis PAGE.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
High quality, high purity and low endotoxin recombinant Recombinant Human Flt3 ligand/Flt3L Protein (RPCB1891), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 0.01 EU/μg of the protein by LAL method
Purity:
≥ 95 % as determined by Tris-Bis PAGE.
Formulation:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Bio-Activity:
Measured in a cell proliferation assay using Human OCI-AML5 cells. The ED 50 for this effect is 1.56-6.24 ng/mL, corresponding to a specific activity of 1.60×10 5 ~6.41×10 5 units/mg.
Reconstitution:
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Recombinant Human Flt3 ligand/Flt3L Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Recombinant Human Flt3 ligand/Flt3L stimulates cell proliferation assay using Human OCI-AML5 cells. The ED50 for this effect is 1.56-6.24 ng/mL, corresponding to a specific activity of 1.60×105~6.41×105 units/mg.
