Isovalerate dehydrogenase [NADP] Cytoplasmic (IDH1) is part of the isocitrate and isopropylmalate dehydrogenases family. The protein IDH1 is a dimer that binds one magnesium or manganese ion per subunit. Metaphyseal chondromatosis with aciduria is observed in patients who have a mutation of IDH1. Glioma occurs as a result of mutations of IDH1. The enzyme IDH is involved in the regeneration of NADPH for intracellular reductions, such as conversion of 2; 4-dienoyl-CoAs to 3-enoyl-CoAs; and peroxisomal reactions that consume 2-oxoglutarate, such as alpha-hydroxylation of phytanic. Human IDH1 Recombinant Protein (R132H, C-8His) is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Human IDH1 Recombinant Protein (R132H, C-8His) (RPES2708)
Recombinant Human Isocitrate Dehydrogenase [NADP] Cytoplasmic is produced by our E.coli expression system and the target gene encoding Met1-Leu414(Arg132His) is expressed with a 8His tag at the C-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
48.1 kDa
AP Mol Mass:
40-50 kDa
Formulation:
Supplied as a 0.2 µm filtered solution of 50mM Tris; 200mM NaCl; 10% glycerol; pH8.0.
Shipping:
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt; store it immediately at<-20°C.
Stability and Storage:
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
