Recombinant Human IL-23 alpha &Mouse IL-12 beta Heterodimer (C- (RPES6211)
Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12. The p19 subunit has homology to the p35 subunit of IL-12, as well as to other single chain cytokines such as IL-6 and IL-11. The p40 subunit is homologous to the extracellular domains of the hematopoietic cytokine receptors. Although p19 is expressed by activated macrophages, dendritic cells, T cells, and endothelial cells, only activated macrophages and dendritic cells express p40 concurrently to produce IL-23. IL-23 has biological activities that are similar to, but distinct from IL-12. Both IL-12 and IL-23 induce proliferation and IFN-gamma production by human T cells. While IL-12 acts on both naive and memory human T cells, the effects of IL-23 is restricted to memory T cells.
Product Name:
Recombinant Human IL-23 alpha &Mouse IL-12 beta Heterodimer (C-
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Calculated MW:
56.9 kDa
Observed MW:
65-80 kDa
Bio-Activity:
Measured by its ability to induce STAT reporter activity in 293F human embryonic kidney cells. The ED50 for this effect is 357.68 ng/ml.
Form:
Lyophilized powder
Source:
HEK293 Cells-derived Human & Mouse IL-23A & IL-12B protein Arg20-Pro189& Met23-Ser335, with an C-terminal His
Formulation:
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Storage:
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80 ℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
