Human IL-2RB; also known asinterleukin-2 receptor subunit beta;is the receptor for interleukin-2. IL2 receptor complex is involved in receptor mediated endocytosis and transduces the mitogenic signals of IL2. IL2 receptor complex has three forms with respect to ability to bind IL2. IL-2RB is belonged to a type I membrane protein;and has a 26 residue signal peptide; a 214 residue extracellular region; a 25 residue transmembrane region and a 286 residue cytoplasmic domain. IL-2RB is the subunit critical for receptor-mediated signaling via physically or functionally coupling to other signaling molecules; such as the Jak-STAT and Src-family protein tyrosine kinase although it lacks apparent catalytic motifs.
Immobilized Human IL-2(Cat: PKSH032629) at 10µg/ml(100 ?l/well) can bind Human IL-2RB-Fc. The ED50 of Human IL-2RB-Fc is 1.37 ug/mL
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Interleukin-2 Receptor subunit beta is produced by our Mammalian expression system and the target gene encoding Ala27-Asp239 is expressed with a Fc tag at the C-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
51.7 kDa
AP Mol Mass:
60-80 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of PBS; pH7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
