Interleukin-2(IL-2) is an interleukin; a type of cytokine signaling molecule in the immune system;belongs to the IL-2 family. It is a potent immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. T-cell proliferation and other fundamental functions that are critical for the immune response require IL-2/IL-2R signaling. IL-2 stimulates growth and differentiation of B-cells; NK cells; lymphokine-activated killer cells; monocytes; macrophages and oligodendrocytes. Interleukin-2/IL-2 Recombinant Protein (C145S) is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Interleukin-2/IL-2 Recombinant Protein (C145S) (RPES1590)
Measured in a cell proliferation assay using CTLL?2 mouse cytotoxic T cells. The ED50 for this effect is 90-270 pg/ml
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Interleukin-2(C145S) is produced by our E.coli expression system and the target gene encoding Pro22-Thr153(Cys145Ser) is expressed.
Purity:
> 95% as determined by reducing SDS-PAGE.
Mol Mass:
15.5 kDa
AP Mol Mass:
14 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 10mM Acetata-Na; 5% Trehaiose; pH 4.5.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
