The Recombinant Human/Mouse/Rat BDNF Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating Recombinant Human/Mouse/Rat BDNF function and its implications in health and disease.
This product (SKU: RPCB1511) is produced using advanced expression systems and features a C-His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 14.35 kDa with an observed molecular weight of 17 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE;≥95 % as determined by HPLC.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
High quality, high purity and low endotoxin recombinant Recombinant Human/Mouse/Rat BDNF Protein (RPCB1511), tested reactivity in E. coli and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 1 EU/μg of the protein by LAL method.
Purity:
≥ 95 % as determined by SDS-PAGE;≥95 % as determined by HPLC.
Formulation:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Bio-Activity:
Immobilized Recombinant Human/Mouse/Rat BDNF Protein at 5μg/mL (100 μL/well) can bind Recombinant Human TrkB with a linear range of 0.38-1.14 μg/mL.2.Immobilized Recombinant Human/Mouse/Rat BDNF Protein at 1μg/mL (100 μL/well) can bind BDNF Rabbit mAb with a linear range of 0.4-2.15ng/mL.3.Immobilized Recombinant Human TrkB at 2μg/mL (100 μL/well) can bind Recombinant Human BDNF with a linear range of 1.95-258ng/mL.
Reconstitution:
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Brain-derived neurotrophic factor (BDNF) is a member of the nerve growth factor family.The neurotrophin family is comprised of at least four proteins including NGF, BDNF, NT-3, and NT-4/5. These secreted cytokines are synthesized as prepropeptides that are proteolytically processed to generate the mature proteins.BDNF cDNA encodes a 247 amino acid residue precursor protein with a signal peptide and a proprotein that are cleaved to yield the 119 amino acid residue mature BDNF. The amino acid sequence of mature BDNF is identical in all mammals examined. BDNF binds with high affinity and specifically activates the TrkB tyrosine kinase receptor.
Recombinant Human/Mouse/Rat BDNF Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
The purity of Recombinant Human/Mouse/Rat BDNF Protein (RPCB1511) was greater than 95% as determined by SEC-HPLC.
Immobilized Recombinant Human/Mouse/Rat BDNF Protein at 1μg/mL (100 μL/well) can bind BDNF Rabbit mAb with a linear range of 0.4-2.15ng/mL.
Immobilized Recombinant Human TrkB at 2μg/mL (100 μL/well) can bind Recombinant Human BDNF with a linear range of 1.95-258ng/mL.
