Description
Recombinant Human NKG2D ligand 2/ULBP2 Protein
The Recombinant Human NKG2D ligand 2/ULBP2 Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating NKG2D ligand 2/ULBP2 function and its implications in health and disease.
This product (SKU: RPCB0383) is produced using HEK293 cells and features a C-hFc&His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 48.52 kDa with an observed molecular weight of 60-70 kDa under denaturing conditions, achieving ≥ 90 % as determined by SDS-PAGE.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
Key Features
| High Purity by Affinity Chromatography | |
| Mammalian & Bacterial Expression Systems | |
| High lot-to-lot consistency via strict QC |
| Product Name: | Recombinant Human NKG2D ligand 2/ULBP2 Protein |
| SKU: | RPCB0383 |
| Size: | 10 μg , 20 μg , 50 μg , 100 μg |
| Reactivity: | Human |
| Synonyms: | ULBP2, ALCAN-alpha, N2DL2, NKG2DL2, RAET1H |
| Tag: | C-hFc&His |
| Expression Host: | HEK293 cells |
| Calculated MW: | 48.52 kDa |
| Observed MW: | 60-70 kDa |
| Gene ID: | 80328 |
| Protein Description: | High quality, high purity and low endotoxin recombinant Recombinant Human NKG2D ligand 2/ULBP2 Protein (RPCB0383), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed. |
| Endotoxin: | < 0.1 EU/μg of the protein by LAL method. |
| Purity: | ≥ 90 % as determined by SDS-PAGE. |
| Formulation: | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. |
| Bio-Activity: | Measured by its binding ability in a functional ELISA. Immobilized Human ULBP2 Protein at 2μg/mL (100 μL/well) can bind NKG2D-raFc with a linear range of 1.95-336.7ng/mL. |
| Reconstitution: | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
| Storage: | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
ULBP2 Protein, Human, Recombinant (His Tag) consists of 203 amino acids with a molecular weight of 23.2 kDa. The apparent molecular mass of recombinant human ULBP2 is about 33 kDa in SDS-PAGE under reducing conditions because of glycosylation.NKG2D ligand 2 is cell membrane protein belonging to theMHC class I family.The gene for ULBP-2 resides in a cluster of ten related genes, six of which encode potentially functional glycoproteins. ULBPs are known to bind to human NKG2D, an activating receptor expressed on NK cells, NKT cells, gamma δ T cells, and CD8+ alpha beta T cells, resulting in the production of cytokines and chemokines. Binding of ULBPs ligands to NKG2D induces calcium mobilization and activation of the JAK2, STAT5, ERK and PI3K kinase/Akt signal transduction pathway.ULBP2 / N2DL-2 is not expressed in normal tissues, but in various types of cancer cell lines and the fetus and has been implicated in tumor surveillance.
 | Recombinant Human NKG2D ligand 2/ULBP2 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue. |
 | Immobilized Human ULBP2 Protein at 2μg/mL (100 μL/well) can bind NKG2D-raFc with a linear range of 1.95-336.7ng/mL. |
