The Recombinant Human PECAM-1/CD31 Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating PECAM-1/CD31 function and its implications in health and disease.
This product (SKU: RPCB0284) is produced using HEK293 cells and features a C-His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 65.30 kDa with an observed molecular weight of 85-105 kDa under denaturing conditions, achieving ≥ 90 % as determined by SDS-PAGE.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
High quality, high purity and low endotoxin recombinant Recombinant Human PECAM-1/CD31 Protein (RPCB0284), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 0.1 EU/μg of the protein by LAL method.
Purity:
≥ 90 % as determined by SDS-PAGE.
Formulation:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Bio-Activity:
Measured by its binding ability in a functional ELISA. Immobilized Human CD31 at 1 μg/mL (100 μL/well) can bind Human CTNNB1 with a linear range of 9.7 ng/mL-1.5 μg/mL.2.Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. When 8×104 cells/well are added to CD31 coated plates (5μg/mL, 100 μL/well) in the presence of 10 μg/mL PHA,approximately 60-70% will adhere after 30 minutes at 37℃. 3.Measured by its binding ability in a functional ELISA.Immobilized APC anti-human CD31 Antibody at 1μg/mL (100 μL/well) can bind Human CD31 Protein with a linear range of 4-25.8 ng/mL.
Reconstitution:
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation.
Recombinant Human PECAM-1/CD31 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Immobilized Human CD31 at 1 μg/mL (100 μL/well) can bind Human CTNNB1 with a linear range of 9.7 ng/ml-1.5μg/mL.
