V-Set and Immunoglobulin Domain-Containing Protein 4 (VSIG4) is a 45-50 kDa macrophage-specific transmembrane glycoprotein that belongs to the B7 family-related protein and an Ig superfamily member. In contrast to the B7 family members which contain two IgG domains; VSIG4 contains one complete V-type Ig domain and a truncated C-type I g domain. VSIG4 is abundantly expressed in several fetal tissues. In adult tissues; the highest expression of VSIG4 is in lung and placenta. It is also expressed in resting macrophages. No VSIG4 expression appears to be present in T and B cells. The specific expression of VSIG4 on resting macrophages in tissue suggests that this inhibitory ligand may be important for the maintenance of T cell unresponsiveness in healthy tissues. VSIG4 functions as a negative regulator of T cell activation; and may be involved in the maintenance of peripheral T cell tolerance; and is also identified as a potent suppressor of established inflammation. VSIG4 is a phagocytic receptor; strong negative regulator of T-cell proliferation and IL2 production. It is a potent inhibitor of the alternative complement pathway convertases. Human VSIG4 is 399 amino acids (aa) in length. It is a type I transmembrane (TM) glycoprotein that contains a 264 aa extracellular domain (ECD) (aa 20 - 283) and a 95 aa cytoplasmic region.
Product Name:
Human VSIG4 Recombinant Protein (RPES2278)
Product Code:
RPES2278
Size:
10µg
Species:
Human
Expressed Host:
Human Cells
Synonyms:
V-Set and Immunoglobulin Domain-Containing Protein 4, Protein Z39Ig, VSIG4, CRIg, Z39IG
Accession:
Q9Y279-1
Sequence:
Arg20-Pro283
Fusion tag:
C-6His
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human V-Set and Immunoglobulin Domain-Containing Protein 4 is produced by our Mammalian expression system and the target gene encoding Arg20-Pro283 is expressed with a 6His tag at the C-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
30.2 kDa
AP Mol Mass:
50 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM PB; 150mM NaCl; pH 7.2.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
