Aminoglycoside 3'-phosphotransferase (APH(3')), also known as aminoglycoside kinase, is an aminoglycoside-modifying enzyme and widely presented in resistant bacteria. These ATP-dependent enzymes phosphorylate the 3'-hydroxyl of a variety of aminoglycosides including kanamycins, neomycins, paromomycins, neamine, ribostamycin, geneticin, and paromamine. These phosphorylated aminoglycosides fail to bind to their respective ribosomal binding sites with high affinity; hence resistance is conferred to the drugs that are phosphorylated. APH(3') is primarily found in certain species of gram-positive bacteria.
Product Name:
Klebsiella pneumoniae NEO Recombinant Protein (RPES3535)
Product Code:
RPES3535
Size:
10µg
Species:
Klebsiella pneumoniae
Expressed Host:
E.coli
Synonyms:
Aminoglycoside 3'-phosphotransferase, APH(3')-II, APH(3')II, Kanamycin kinase type II, Neomycin-kanamycin phosphotransferase type II, neo
Accession:
P00552
Sequence:
Met1-Phe264
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Klebsiella pneumoniae NEO is produced by our E.coli expression system and the target gene encoding Met1-Phe264 is expressed.
Purity:
> 95% as determined by reducing SDS-PAGE.
Mol Mass:
29 kDa
AP Mol Mass:
28-30 kDa
Formulation:
Supplied as a 0.2 µm filtered solution of PBS, pH 7.4, 20% Glycerol.
Shipping:
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt, store it immediately at<-20°C.
Stability and Storage:
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
