The Recombinant Mouse Apolipoprotein E/APOE Protein is a high-quality recombinant protein designed for murine biological research applications. This protein serves as an essential reagent in mouse model studies, comparative immunology research, and preclinical therapeutic evaluations, enabling scientists to investigate Apolipoprotein E/APOE biology and its relevance to human disease mechanisms through translational research approaches.
This product (SKU: RPCB0665) is produced using HEK293 cells and features a C-His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 34.81 kDa with an observed molecular weight of 35-45 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
High quality, high purity and low endotoxin recombinant Recombinant Mouse Apolipoprotein E/APOE Protein (RPCB0665), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 1 EU/μg of the protein by LAL method.
Purity:
≥ 95 % as determined by SDS-PAGE.
Formulation:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Bio-Activity:
Measured by its binding ability in a functional ELISA. Immobilized Mouse APOE(RPCB0665 ) at 5μg/mL (100 μL/well) can bind Mouse TREM2(RPCB1621) with a linear range of 0.16-1.5μg/mL.
Reconstitution:
Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Apolipoprotein E (Apo-E), is a member of the apolipoprotein A1/A4/E family. ApoE is a major proteincomponent of serum LDL, VLDL, HDL, and chylomicrons. APOE may function in mediating the binding,internalization, and catabolism of lipoprotein particles. It can serve as a ligand for the LDL (apo B/E) receptorand for the specific apo-E receptor (chylomicron remnant) of hepatic tissues. APOE is usually secreted inplasma. Phosphorylation sites are present in the extracellular medium.
Recombinant Mouse Apolipoprotein E/APOE Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Immobilized Mouse APOE(RPCB0665 ) at 5μg/mL (100 μL/well) can bind Mouse TREM2(RPCB1621) with a linear range of 0.16-1.5μg/mL.
