The Recombinant Mouse IL-1 alpha Protein is a high-quality recombinant protein designed for murine biological research applications. This protein serves as an essential reagent in mouse model studies, comparative immunology research, and preclinical therapeutic evaluations, enabling scientists to investigate IL-1 alpha biology and its relevance to human disease mechanisms through translational research approaches.
This product (SKU: RPCB1917) is produced using E. coli and features a NO-Tag tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 17.99 kDa with an observed molecular weight of 15-25 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE;≥ 95 % as determined by HPLC.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
High quality, high purity and low endotoxin recombinant Recombinant Mouse IL-1 alpha Protein (RPCB1917), tested reactivity in E. coli and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 0.1 EU/μg of the protein by LAL method.
Purity:
≥ 95 % as determined by SDS-PAGE;≥ 95 % as determined by HPLC.
Formulation:
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Bio-Activity:
Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 for this effect is 3.075-12.3 pg/mL, corresponding to a specific activity of 8.13×107~3.25×108 units/mg.
Reconstitution:
Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Interleukin 1 alpha (IL-1α) also known as hematopoietin 1 is a cytokine of the interleukin 1 family that in humans is encoded by the IL1A gene. IL-1α is produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells. It possesses metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. It binds to the interleukin-1 receptor. It is on the pathway that activates tumor necrosis factor-alpha.
Recombinant Mouse IL-1 alpha Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Recombinant Mouse IL-1 alpha Protein is greater than 95% as determined by SEC-HPLC.
