Description
| SKU: | RPCB2540 |
| Size: | 10 μg, 20 μg, 50 μg, 100 μg |
| Tag: | C-His |
| Reactivity: | Mouse |
| Protein Description: | Recombinant Mouse IL-17F Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met1-Ala161) of mouse IL-17F (Accession #NP_665855.2) fused with a 6×His tag at the C-terminus. |
| Endotoxin: | < 0.1 EU/μg of the protein by LAL method. |
| Purity: | ≥ 95 % as determined by SDS-PAGE;≥ 95 % as determined by HPLC. |
| Formulation: | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. |
| Gene ID: | 257630 |
| Storage: | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
| Reconstitution: | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
| Swiss-Prot: | NP_665855.2 |
 | Recombinant Mouse IL-17F Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue. |
 | Immobilized recombinant Mouse IL-17F at 2μg/mL (100μL/well) can bind recombinant Mouse IL17RA with a linear range of 31. 25-163ng/mL. |
 | Measured by its ability to induce IL-6 secretion by NIH-3T3 mouse embryonic fibroblast cells in the presense of 2 ng/mL TNFα(Catalog: RPCB0855). The ED50 for this effect is 10. 18-140.72 ng/mL, corresponding to a specific activity of 2. 46×10 4~9. 82×10 4 units/mg. |
