The Recombinant Rat 5'-Nucleotidase/NT5E/CD73 Protein is a high-quality recombinant protein produced for advanced research applications in molecular biology and biotechnology. This protein serves as a critical reagent in various experimental contexts, including functional studies, binding assays, and therapeutic development programs, providing researchers with a standardized and reliable tool for investigating protein function and interactions.
This product (SKU: RPCB0676) is produced using HEK293 cells and features a C-6×His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 59 kDa with an observed molecular weight of 70-90 kDa under denaturing conditions, achieving ≥ 90 % as determined by SDS-PAGE., ensuring exceptional quality and consistency for research applications.
High quality, high purity and low endotoxin recombinant Recombinant Rat 5'-Nucleotidase/NT5E/CD73 Protein (RPCB0676), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 1 EU/μg of the protein by LAL method.
Purity:
≥ 90 % as determined by SDS-PAGE.
Formulation:
Lyophilized from a 0.2 μm filtered solution of PBS,pH7.4.Contact us for customized product form or formulation.
Reconstitution:
Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
CD73, also known as Ecto-5'-nucleotidase, is an ectoenzyme that is attached to the cell membrane by a glycosylphophatidylinositol anchor. The enzyme is expressed by most cell types. The 5 ’ -Nucleotidase activity of CD73converts extracellular nucleoside-5 ’ -monophosphates to nucleosides. CD73 is one of several enzymesresponsible for the production of extracellular adenosine, a signaling molecule that is involved in responses toinflammation and tissue injury. CD73 hydrolyzes extracellular nucleotides into membrane permeablenucleosides.
Recombinant Rat 5'-Nucleotidase/NT5E/CD73 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
